Part:BBa_K4140012

Human U6 promoter

Part Description

A proximal sequence element, which attracts the multisubunit component SNAPc, and a TATA box, which recruits the TATA box-binding protein, TBP, make up the human U6 small nuclear RNA core promoter. Two clearly identified factors are necessary for transcription from the human U6 promoter in addition to SNAPc and TBP. The first is the human homologue of the yeast TFIIIB subunit B" (hB), which is generally necessary for the transcription of RNA polymerase III genes, and the second is the human homologue of the TFIIIB subunit BRF (hBRFU), which is one of two human homologues and is specifically necessary for the transcription of U6-type RNA polymerase III promoters.

Usage

Human U6 small nuclear RNA core promoter.formed of Two clearly identified factors are necessary for transcription, A proximal sequence element, which attracts the multisubunit component SNAPc, and a TATA box, which recruits the TATA box-binding protein, TBP,Its used to regulate the expression of our regulatory system that is formed of two domains cas12g protein and it’s gRNA which is responsible for tuning of our circuit to prevent over expression of PAH.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]