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Part:BBa_K4140007

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 18:09, 28 September 2022 by Ahmed Wael (Talk | contribs) (→‎Part Description)


kp-sp


Part Description

KP-SP is a secreted signal peptide at the N-terminus of the reporter protein that enables it's secretion extracellularly. this peptide comes from E.Coli as well as actinomycetes and can be secreted under normal instances

Usage

Signal at the N-terminus of the reporter protein to transmit it from intracellular to extracellular we link that signal to PAH for transmitting it extracellularly to hydroxylate excess phenylalanine into tyrosine.

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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