Coding

Part:BBa_K4140001

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 08:24, 29 September 2022 by Mohamed-Ab0elghare (Talk | contribs) (→‎Usage)


TyrR


Part Description

The TyrR protein in Escherichia coli has the ability to both activate and repress the transcription operons necessary for the production and intake of aromatic amino acids (tyrosine, phenylalanine, and tryptophan). As an illustration, the TyrR dimer activates the promoter of the tyrP gene, which codes for a transporter specific to tyrosine, when tyrosine is present

Usage

We took advantage of The TyrR protein properties and it’s ability to control the biosynthesis and intake of aromatic amino acids (tyrosine, phenylalanine, and tryptophan). So we employ it to control the activity of the paroF promoter and TyrP promoter as  TyrR dimer activates the TyrP promoter and paroF promoter in the presence of phenylalanine , when tyrosine is more abundant  the induction effect on paroF is lost

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 720
    Illegal BglII site found at 1062
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 526
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None