Regulatory
Part:BBa_K4387000
Designed by: Jana Mehdy, Lea Bruellmann, Marine Mausy Group: iGEM22_UZurich (2022-09-28)
Nitric Oxide Sensing Promoter pNorVβ
PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and norR, the corresponding transcription factor. IHF was removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/basic_part/winner
None |