RNA

Part:BBa_K3743020

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM21_AFCM-Egypt   (2022-09-24)
Revision as of 09:55, 28 September 2022 by Ahmed A Rezk (Talk | contribs)


VSVg-Fusogen


Part Description

It is the RNA domain of CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle

Usage

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1396
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 987
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None