![](https://parts.igem.org/images/partbypart/icon_translational_unit.png)
Translational_Unit
tphB
Part:BBa_K4378000:Design
Designed by: Leander Maximilian Linke Group: iGEM22_Uppsala (2022-09-28)
TphB: An effective overexpression casette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 288
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 409
Design Notes
For potential in vitro use of the enzyme an T7-Tag is attached to allow purification through affinity chromatography.
Source
https://www.uniprot.org/uniprotkb/Q3C1E1/entry