Translational_Unit
tphB

Part:BBa_K4378000

Designed by: Leander Maximilian Linke   Group: iGEM22_Uppsala   (2022-09-28)
Revision as of 13:25, 28 September 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


TphB: An effective overexpression casette

TphB or 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase (DCDDH) is an enzyme involved in the degradation of terephtalate (TPA). This enzyme plays therefore a key role in the degradation of polyethylenterephthalate (PET) into protocatachuic acid (PCA). The precise reaction performed is the decarboxylation of 1,6-dihydroxycyclohexa-2,4-diene-dicarboxylate (DCD) into PCA. This enzyme is known to assemble as a homodimer whith each unit adapting to an alpha-beta fold with an mixed beta sheet active site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 409


[edit]
Categories
//cds/transcriptionalregulator/repressor
//chassis/prokaryote/ecoli
//proteindomain/affinity
Parameters
chassisE. coli