Coding
Part:BBa_K4140004
Designed by: Ahmed Gamal Mohamed Mattar Group: iGEM22_AFCM-Egypt (2022-09-24)
P2A linker
In order to mediate the "cleavage" of two proteins, 2A peptide sequences were discovered in Picornaviruses. Effective multiple protein expression from a single open reading frame (ORF) is achieved using 2A peptide-linked multicistronic vectors. Use alternative 2A peptide sequences when linking more than two genes to reduce the likelihood of homologous recombination. All eukaryotic systems studied to date, including mammalian cells, yeast, and plants, have effectively utilised the 2A peptide system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |