Coding
leghemoglo

Part:BBa_K4232001

Designed by: Ruisi Chen   Group: iGEM22_BIT-China   (2022-09-23)
Revision as of 02:13, 28 September 2022 by Eatting (Talk | contribs)


It is the "switch" that regulates the oxygen concentration in the rhizosphere

This part of the code is leghemoglobin. It comes from the soybean rhizosphere. It can bind to intracellular oxygen and transport it to the end of the aerobic respiratory chain (such as the cell membrane of E. coli), thus creating a localized hypoxic environment in the cell, yet leaving the process of aerobic respiration providing energy unimpaired (The specific mechanism of action is shown in the figure ).
BIT-China-parts-leghemoglobin-mechanism.jpeg

Since the bean hemoglobin gene is of plant origin, when performing gene recombination on prokaryotes, it is necessary to remove introns from their gene sequences, do codon optimization, and take measures to make them active after expression.

Reference：Andrés R. Schwember, et al.Regulation of Symbiotic Nitrogen Fixation in Legume Root Nodules.(2019)Plants.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None