Part:BBa_K4324001

lacPO-RBS-XDH-T1

This part allows E. coli to express xylitol dehydrogenase (XDH).

XDH is an enzyme that allows the conversion of xylitol to xylulose, and vice versa, according to the following chemical equation: xylitol + NAD+ ⇌ D-xylulose + NADH + H+

In S. cerevisiae yeast cells, XDH forms the second process in the XR-XDH pathway, which converts xylose into xylulose via xylitol. Xylulose is then converted into xylulose-5-phosphate (X5P) for further metabolism in the pentose phosphate pathway.

E. coli do not exhibit the XR-XDH pathway, instead having an XI pathway which directly converts xylose into xylulose. Hence, together with xylose reductase (XR) which can convert xylitol to xylulose, XDH presents an alternate xylose metabolism pathway for E. coli.

Furthermore, this part enables E. coli to utilise xylitol as an energy source through its conversion to xylose, which then follows the XI pathway.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 700
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 357