Coding
HlyB

Part:BBa_K4387999

Designed by: Nathalie Weibel   Group: iGEM22_UZurich   (2022-09-27)
Revision as of 20:26, 27 September 2022 by Registry (Talk | contribs)

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HlyB of the hemolysin A one-step secretion system

The hemolysin A secretion machinery is a one-step secretion system (T1SSI) that was originally isolated from uropathogenic E. coli strains. It is composed of 3 main peptides, the inner membrane proteins HlyB and HlyD, and the outer membrane protein TolC. Together, these 3 proteins build a continuous channel, through which originally the HlyA toxin is secreted in a one-step manner. Interestingly, the secretion signal is not found on the N-terminal site, instead it is found at the C-terminal end and the signal sequence is not removed during secretion. Scientists have identified the secretion signal and by fusing it to different proteins, were able to secrete various proteins with this secretion machinery.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1096
    Illegal AgeI site found at 1266
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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