Composite

Part:BBa_K4235029

Designed by: Maulik Masaliya   Group: iGEM22_Stony_Brook   (2022-09-21)
Revision as of 22:16, 21 September 2022 by Maulik masaliya (Talk | contribs) (→‎Usage and Biology)


Ampicillin resistance gene cassette

Usage and Biology

This part is the entire circuit of the Ampicillin Resistance gene which is used within the plasmid and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter BBa_K4235017. This marker was used for the selection of successful transformants after cloning our gene insert BBa_K4235011 in the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 647
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 647
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 647
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 821


[edit]
Categories
//cds
//cds/selectionmarker/antibioticresistance
//chassis/prokaryote
Parameters
None