Device
Part:BBa_K302018
Designed by: Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall Group: iGEM10_Newcastle (2010-08-18)
Revision as of 15:13, 28 September 2010 by Swoodhouse (Talk | contribs)
Subtilin immunity
Subtilin is a lantibiotic peptide produced naturally by Bacillus subtilis ATCC 6633 but not by Bacillus subtilis 168.
This part encodes two separate subtilin immunity systems — a membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores, and a transporter which removes subtilin from the membrane — which together provide a high degree of subtilin resistance.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 837
Illegal BglII site found at 1585 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1852
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1132
Illegal SapI site found at 1412
Illegal SapI.rc site found at 2135
[edit]
Categories
Parameters
None |