Part:BBa_K4268000:Design
S-TIP37 Head-Tail Connector
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 346
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Synechococcus sp PCC 11901, was decided as the desired cyanobacterial species that SUNY_Oneonta iGEM would like to focus on? S-TIP 37 is the infecting cyanophage of this cyanobacterial species. However, the genome has not undergone full sequencing and many of its genes remain hypothetical proteins. Thus, in comparison to Syn-5, another T7-like phage that has been fully sequenced, was needed. Both S-TIP 37 and Syn-5 are structurally and genetically similar allowing the team to distinguish the genes needed for producing the phage shell.
Insert poster table comparison
Upon the isolation of the Head-Tail Connector sequence, the following edits were made:
- Restriction sites were removed/altered to ensure RCF10 and RCF1000 compatibility
- To complete Level 0 Golden Gate Assembly, the sequence contains SapI sites at the ends
Source
The source of this sequence is from the S-TIP37 genome (GenBanK: NC_048026.1)
References
The S-TIP37 genome was found on GenomeNet