DNA

Part:BBa_K4223012

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-25)
Revision as of 16:50, 25 September 2022 by Trk840223331 (Talk | contribs)


DNA template of crRNA for Cas13a1 in vitro detection system

5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3'
crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the BBa_K4223001,BBa_K4223009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None