Coding
Part:BBa_K4202004:Experience
Designed by: JUNYI LIU Group: iGEM22_ZJU-China (2022-09-25)
Revision as of 13:37, 25 September 2022 by Eeeeeartha (Talk | contribs) (→Expression of BBa_K4202004)
Expression of BBa_K4202004
After chemical transformation of plasmid with this part, the transformed Bacillus subtilis WB600 were cultured in optimized LB and SMM medium, and obtained crude enzyme solution by centrifugation and ultrasonic disruption.Then we detected the molecular mass by SDS-PAGE and coomassie blue staining.
SDS-PAGE displayed bands of 37kDa and 74kDa for CA monomer and dimer, which didn' t exist in the control group(Fig.1-1)
Applications of BBa_K4202004
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UNIQ8ff74f9561256ab7-partinfo-00000000-QINU UNIQ8ff74f9561256ab7-partinfo-00000001-QINU