Part:BBa_K4175001

Notch core

This Notch core domain is derived from the transmembrane part of the Notch receptor.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 115
  Illegal PstI site found at 156
  Illegal PstI site found at 202
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 115
  Illegal PstI site found at 156
  Illegal PstI site found at 202
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 115
  Illegal PstI site found at 156
  Illegal PstI site found at 202
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 115
  Illegal PstI site found at 156
  Illegal PstI site found at 202
  Illegal NgoMIV site found at 28
  
• 1000
  COMPATIBLE WITH RFC[1000]

Biology

Notch receptors are large single-pass Type I transmembrane proteins, which contain 29-36 tandem epidermal growth factor (EGF)-like repeats in their extracellular domain. In mammals, there are four Notch paralogs (mNotch1-4) (Kopan and Ilagan, 2009). The Notch signaling pathway, mediating short-range cell-cell communication, is crucial for metazoan development and tissue regeneration. For this reason, this signaling pathway is conserved among species (Kopan and Ilagan, 2009). The Notch receptors could bind to their ligands (e.g., Delta in drosophila, Dll1 in mammals) either on the neighboring cells or on the same cell where the Notch receptors are expressed. When the Notch receptor interact with its ligand through extracellular EGF-like repeats, the receptor will undergo two subsequent cleavages by ADAM metalloproteases and γ-secretase. As a result, the intracellular domain of Notch receptor is released, enters the nucleus, and acts with DNA-binding protein CSL to activate transcription (Kopan and Ilagan, 2009) (Fig 1). This allows regulation of differentiation, proliferation, and apoptotic events of cells at all stages of development (Artavanis-Tsakonas et al., 1999).