Coding
Part:BBa_K4175000
Designed by: Li Xianxiu, Zhang Wanying Group: iGEM22_ZJUintl-China (2022-08-27)
anti-IL-6 scFv
Biology
Anti-IL-6 single chain variable fragment (scFv) can bind to interleukin 6 (IL-6). It comprises of a variable heavy chain domain and a variable light chain domain, with a GSG linker joining the two domains. The variable heavy and light chain domain are derived from the human anti-IL-6 monoclonal antibody AME-19a [1].
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 604
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 604
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 512
Illegal XhoI site found at 496 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 604
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 604
Illegal AgeI site found at 556 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
biology | Human |