DNA

Part:BBa_K4115010:Design

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-21)
Revision as of 16:23, 21 September 2022 by Yurj (Talk | contribs) (→‎Source)


NSI-US


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 333
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NSI-US is placed upstream of the aimed fragments when constructing our plasmids needed for HL7942 and UTEX2973.


Source

The sequence is gained from the genome of Synechococcus UTEX2973 and the fragment we use is synthesized by the company.

References