Translational_Unit

Part:BBa_K4223003

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-20)
Revision as of 08:39, 20 September 2022 by Trk840223331 (Talk | contribs)


Transcription system of target RNA for LbCas13a

Cas13a tolerates one or two mismatches between crRNA and the target sequence, which will result in a greatly reduced cleavage efficiency of Cas13a protein, and this wrong cleavage will also lead to "false positives" in the detection.
In order to confirm the occurrence of false positives, Hainanu-China designed a series of sequences with single nucleotide polymorphisms (SNPS) based on the target RNA, which were detected by fluorescence reporter.
It should be noted that fluorescence was generated by trans-cleavage of ssRNA cleavage Reporter after Cas13a recognized and cleaved the target RNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 100
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None