Device

Part:BBa_K176031:Design

Designed by: Chao Li, Danqian Liu, Hao Jiang   Group: iGEM09_USTC   (2009-07-20)
Revision as of 14:06, 20 July 2009 by Registry (Talk | contribs)

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Repressible AHL->PoPS Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet->PoPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 797
    Illegal NheI site found at 820
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1775


Design Notes

Input1: PoPS

Input2: AHL

Output: PoPS

Design:

Input1 PoPS generate TetR(no LVA)

pCon 0.70(J23101) generate LuxR

pLux/Tet(K176000) generate Output PoPS, activated by LuxR and Input2 AHL, repressed by TetR and no aTc


Source

K176001 K176028

References