Coding

Part:BBa_K4390012:Design

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-08-07)
Revision as of 12:10, 7 August 2022 by Registry (Talk | contribs)

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Danio rerio Metallothionein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimised for expression in E. coli K12. The native stop codon has been removed and replaced with a double stop codon.



Source

Yan, C.H.M., Chan, K.M., 2004. Cloning of zebrafish metallothionein gene and characterization of its gene promoter region in HepG2 cell line. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. https://doi.org/10.1016/j.bbaexp.2004.04.004

References