Composite

Part:BBa_K4370010:Design

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-17)
Revision as of 17:03, 21 August 2022 by Sburymone (Talk | contribs)


STREPTO_E.coli_CLONING_MODULE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). This module is also designed to be easily cloned between NotI sites in the collection of Streptomyces integrative vectors published by Aubry and collaborators (Appl Environ Microbiol, 2019).


Source

The sequence has been synthetized.

References

Chaoxian Bai , Yang Zhang , Xuejin Zhao, Yiling Hu, Sihai Xiang, Jin Miao, Chunbo Lou, Lixin Zhang “Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces”, Proc Natl Acad Sci U S A , 2015 Sep 29;112(39):12181-6. https://www.pnas.org/doi/10.1073/pnas.1511027112

Weishan Wang, Xiao Li, Juan Wang, Sihai Xiang, Xiaozhou Feng, Keqian Yang, “An engineered strong promoter for Streptomycetes”, Appl Environ Microbiol., 2013 Jul;79(14):4484-92. https://doi.org/10.1128/AEM.00985-13

Céline Aubry, Jean-Luc Pernodet , Sylvie Lautru, « Modular and Integrative Vectors for Synthetic Biology Applications in Streptomyces spp”, Appl Environ Microbiol, 2019 Aug 1;85(16):e00485-19 https://doi.org/10.1128/AEM.00485-19