Composite

Part:BBa_K4370010:Design

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-17)
Revision as of 13:39, 18 August 2022 by Sburymone (Talk | contribs)


STREPTO_E.coli_CLONING_MODULE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). This module is also designed to be easily cloned between NotI sites in the collection of Streptomyces integrative vectors published by Aubry and collaborators (Appl Environ Microbiol, 2019).


Source

The sequence has been synthetized.

References

Bai et al., PNAS, 2015 - www.pnas.org/cgi/doi/10.1073/pnas.1511027112

Wang et al., Appl Environ Microbiol, 2013 - https://journals.asm.org/doi/10.1128/AEM.00985-13

Aubry et al., Appl Environ Microbiol, 2019 - https://journals.asm.org/doi/10.1128/AEM.00485-19