Composite

Part:BBa_K4370010

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-17)
Revision as of 16:02, 17 August 2022 by Registry (Talk | contribs)

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KasOP*_promoter_STREPTO

This part corresponds to a cloning module allowing the cloning of gene of interest under the control of the Streptomyces constitutive strong promoter KasOP* between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). Between these two sites is inserted an expression cassette for the expression of mRFP1 (BBa_E1010) under the control of an E. coli promoter <i/>. Therefore this module allows the easy red/white cloning of sequences of interest in place of the E. coli expression module.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None