Coding
cbbL_STRBO

Part:BBa_K4370000:Design

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-17)
Revision as of 14:36, 17 August 2022 by Sburymone (Talk | contribs)


cbbL_STRBO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 373


Design Notes

The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli .


Source

This sequence has be synthetized.

References