Regulatory

Part:BBa_K145150

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-06)
Revision as of 12:36, 14 October 2008 by BNathalie (Talk | contribs)

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed

Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; OR2 and OR1 located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/multiple
//regulation/negative
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
negative_regulators1
positive_regulators1