Coding

Part:BBa_K4150004

Designed by: WEI CHIEH CHIN   Group: iGEM22_Mingdao   (2022-08-11)
Revision as of 08:17, 11 August 2022 by Jacky CHIN (Talk | contribs)


His-OVA

The chicken ovalbumin sequence obtained from KEGG database (Gallus gallus) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 373


[edit]
Categories
//awards/basic_part/nominee
Parameters
None