Composite

Part:BBa_J70310:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-06-16)
Revision as of 20:23, 16 June 2009 by Registry (Talk | contribs)

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Mesoplasma florum pBG7AU plasmid origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 99
    Illegal NheI site found at 120
    Illegal NheI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Unclear location for the cut site of the circular plasmid.



Source

PCR from recircularized pBG7AU cut at the ClaI site. Original plasmid from Djordjevic01. Primers:

GTTTCTTC GAATTC GCGGCCGC T TCTAGA Ggagtaaattctcctttttttgg,BG7AU-F

GTTTCTTC CTGCAG CGGCCGC T ACTAGT Acaaaaaaaggagaatttactcc,BG7AU-R


References