Coding

Part:BBa_K4161002

Designed by: Chang Shen   Group: iGEM22_DKU   (2022-07-20)
Revision as of 08:54, 20 July 2022 by Chang Shen (Talk | contribs)


JMK-H2

JMK-H2 is a VHH antibody wich targets the IpaD antigen that makes up the needle tip structure of Type III secretion system of Shigella. The DNA sequence is reversed from the JMK-H2 protein sequence and codon optimized for expression in E. coli.

Source

Barta, Michael L., Jonathan P. Shearer, Olivia Arizmendi, Jacqueline M. Tremblay, Nurjahan Mehzabeen, Qi Zheng, Kevin P. Battaile, et al. “Single-Domain Antibodies Pinpoint Potential Targets within Shigella Invasion Plasmid Antigen D of the Needle Tip Complex for Inhibition of Type III Secretion.” Journal of Biological Chemistry 292, no. 40 (October 2017): 16677–87. https://doi.org/10.1074/jbc.M117.802231.

The sequence can be accessed through RCSB PDB with PDB ID 5VXK. (https://www.rcsb.org/structure/5VXK)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None