Coding

Part:BBa_K4161001

Designed by: Chang Shen   Group: iGEM22_DKU   (2022-07-20)
Revision as of 07:51, 20 July 2022 by Chang Shen (Talk | contribs)


20IpaD

20IpaD is a VHH antibody wich targets the IpaD antigen, which makes up the needle tip of Type III secretion system of Shigella. The DNA sequence is reversed from the 20IpaD protein sequence and codon optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
    Illegal PstI site found at 318
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None