Composite

Part:BBa_K4347011:Design

Designed by: Victor Di Donato, Nicoletta de Maat   Group: iGEM22_Queens_Canada   (2022-07-07)
Revision as of 15:56, 8 July 2022 by Victor5688 (Talk | contribs)


Bst fusion with Sac7e and point mutations for enhanced thermal stability codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Research that lead to choosing sac7e as ssb protien. Alpha fold analysis Yasara analysis

Considerations

Section can be summary from https://parts.igem.org/Part:BBa_K4347007# and https://parts.igem.org/Part:BBa_K4347010. -Considerations with point mutations. - Previous work that had already been done, heating mechanism of our device (might be more than 65 bc its a kettle based device), how fusion would interfere bst structure eg, alpha fold with phi29.

Source

Bst: https://parts.igem.org/Part:BBa_K3790000 Sac7e: https://parts.igem.org/Part:BBa_K4347006

References