Composite

Part:BBa_K4421027

Designed by: Shuyang Zhu   Group: iGEM22_NMU_China   (2022-07-14)
Revision as of 04:05, 16 July 2022 by Juejue (Talk | contribs)


CAR_library

As we have mentioned in BBa_K4421003, we designed a scFv library with 10 individual colonies. This composite part is formed of 10 kinds of unique CAR constructs based on BBa_K4421003.

Special Note

Since our part BBa_K4421003 is a general concept without a specific sequence, we use BBa_K4421011 cetuximab scfv to serve as a placeholder. In other words, the place where BBa_K4421011 at can be replaced by any other scFv to form diverse CAR constructs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 1832
    Illegal NgoMIV site found at 1898
    Illegal NgoMIV site found at 2065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1439
    Illegal SapI.rc site found at 1996


[edit]
Categories
Parameters
None