Composite

Part:BBa_K4347011:Design

Designed by: Victor Di Donato, Nicoletta de Maat   Group: iGEM22_Queens_Canada   (2022-07-07)
Revision as of 15:43, 8 July 2022 by Victor5688 (Talk | contribs)


Bst fusion with Sac7e and point mutations for enhanced thermal stability codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Research that lead to choosing sac7e as ssb protien. Alpha fold analysis Yasara analysis

Considerations

section can be summary of

Considerations with point mutations.

Previous work that had already been done, heating mechanism of our device (might be more than 65 bc its a kettle based device), how fusion would interfere bst structure eg, alpha fold with phi29.

Source

Bst: https://parts.igem.org/Part:BBa_K3790000 Sac7e: https://parts.igem.org/Part:BBa_K4347006

References