Part:BBa_K4421010

2A sequence

The 2A peptide sequence self-cleaving sequence， was intercalated between iCASP9 and the GFP tag and it is derived from FMDV (foot-and-mouth disease virus) to allow transcription and expression of one single mRNA molecule.

Usage and Biology

The 2A self-cleaving peptide was first identified in 1991 from foot-and-mouth disease virus(Ryan et al., 1991). The 2A peptide, containing the canonical motif DxExNPGP (where “x” is any amino acid which is not conserved), induces the recombinant protein containing 2A peptide to shear itself in the cell, often at the site corresponding to the "-NPG/P-" junction(DOI: 10.5661/bger-26-223). In some studies, the 2A peptide was used to link icasp9 gene with cytokine and leucocyte differentiation antigen gene(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2018664/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236370/),thus 少些质粒。 So we designed it to link icasp9 and GFP, . Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]