Regulatory

Part:BBa_K4421021

Designed by: Shuyang Zhu   Group: iGEM22_NMU_China   (2022-06-03)
Revision as of 05:29, 7 June 2022 by Juejue (Talk | contribs)


NFAT-RE promotor

This part is a CAR inducible promoter containing six NFAT-REs in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None