Composite

Part:BBa_K3893011:Design

Designed by: Sharon Ortiz   Group: iGEM21_Ecuador   (2021-10-21)
Revision as of 01:34, 22 October 2021 by Registry (Talk | contribs)

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Down part of cassette for dsRNA constitutive production 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 800
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 800
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 800
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 128


Design Notes

It isn't compatible with RFC10 Assembly. Because the XbaI and KpnI restriction sites are needed in the middle of the sequence for the assembly system.


Source

An assembly set of basic parts

References