Regulatory

Part:BBa_K3893000:Design

Designed by: Arly Armas   Group: iGEM21_Ecuador   (2021-10-20)
Revision as of 00:27, 22 October 2021 by Acarmas6 (Talk | contribs) (→‎References)


Shine Dalgarno for E coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that the part is suitable with the assembly RFC10. Therefore, the sequence does not have restriction recognition sites for the enzymes: EcoRI, XbaI, SpeI, PstI and NotI

Source

It comes from the original SD sequence reported for E.coli

References

[1] A. Yurovsky, M. R. Amin, J. Gardin, Y. Chen, S. Skiena, y B. Futcher, “Prokaryotic coding regions have little if any specific depletion of Shine-Dalgarno motifs”, PLOS ONE, vol. 13, núm. 8, p. e0202768, ago. 2018, doi: 10.1371/journal.pone.0202768.