![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Pre B2
Part:BBa_K4060105:Experience
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-09-30)
Revision as of 14:24, 7 December 2021 by Clairechen (Talk | contribs)
Assembly of PreB2 (pSB1C3)
The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS BBa_K115002. We digested the vector fragment BBa_K115002 with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.
![](https://2021.igem.org/wiki/images/e/e1/T--NYCU-Taipei--image4.png)
Fig.6 Agarose electrophoresis in order to verify Pre B2. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B2 digested with NotI.
Applications of BBa_K4060105
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