Composite
Pre B1
Part:BBa_K4060104:Experience
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-09-30)
Revision as of 14:19, 7 December 2021 by Clairechen (Talk | contribs) (→Assembly of Pre B1 (pSB1C3))
Assembly of Pre B1 (pSB1C3)
The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.
Fig.1 Agarose electrophoresis in order to verify Pre B1. Lane 1, 100bp ladder.Lane 1’, partial of Life 1 kb Plus ladder. Lane 2,3, Pre B1 digested with NheI and PstI.
Applications of BBa_K4060104
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