Composite
Pre B1
Part:BBa_K4060104:Experience
Designed by: YU CHUAN LEE Group: iGEM21_NYCU-Taipei (2021-09-30)
Revision as of 14:15, 7 December 2021 by Clairechen (Talk | contribs)
Assembly of Pre B1 (pSB1C3)
The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.
Fig.5 Agarose electrophoresis in order to verify Pre B1.
Applications of BBa_K4060104
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