![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
pJ102
Part:BBa_K4060112
Designed by: CHEN YAN-ZHEN Group: iGEM21_NYCU-Taipei (2021-10-01)
pJ102 is one of our tandem promoter design
K206000 + J23102
These tandem promoters are composed of the pBad(BBa_K206000) and a constitutive promoter with the RFP gene on the downstream. We digested the vector fragment BBa_K206000 with PstI and SpeI and the insert fragment constitutive promoter with RFP by XbaI and PstI. We ligated these fragments together to form pJ102, pJ118, pJ110, pJ116, and pJ113.
![](https://2021.igem.org/wiki/images/2/2b/T--NYCU-Taipei--pJ116PJ118PJ112PJ113PJ110.png)
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