Coding
MazF
Part:BBa_K4060001:Experience
Designed by: Yi Rong Chen Group: iGEM21_NYCU-Taipei (2021-09-30)
Revision as of 13:28, 7 December 2021 by Clairechen (Talk | contribs) (→1. Cloning MazE and MazF gene: The MazE and MAzF have been amplified by PCR from E. coli MG1655 genome DNA.)
1. Cloning MazE and MazF gene
The MazE and MAzF have been amplified by PCR from E. coli MG1655 genome DNA
Fig.1 Agarose electrophoresis in order to verify MazE and MazF genes. The purified DNA fragments had the expected length and were subsequently used for cloning. Lane 1, 100bp ladder. Lane 2, MazE PCR product. Lane 3 and 4, MazF PCR product. Land 5, 100bp ladder.
2. Gibson Assembly:
Fig.2 Agarose electrophoresis in order to verify Construct 1 and Construct 2. Lane 1, 100bp ladder. Lane 1' partial of Life 1 kb Plus ladder. Lane 2 and 3, Construct 1 digested with BamHI. Lane 4, Construct 2 digested with KpnI and NdeI.
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UNIQ38c8fafb8ed10a61-partinfo-00000002-QINU UNIQ38c8fafb8ed10a61-partinfo-00000003-QINU