Part:BBa_K3714010
Improved gard-337 for lateral flow assay
To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary (Figure.1). However, designing the toehold and probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor.
The toehold site in part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme(Figure.2, also see the unchanged BBa_K3714002), the toehold worked as our expect as well (Figure.2).
The stop oligo significantly increased the percentage of the uncleaved band, resembling the effect of gardiquimod (the inhibitor of this aptamer). Thus our engineering is successful.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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