Composite

Part:BBa_K3738013:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 03:38, 22 October 2021 by Marklea (Talk | contribs) (→‎Source)


MS2 Bacteriophage Maturation Protein A with N-Terminal Histidine Tag and TEV Site (Composite)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 646
    Illegal BamHI site found at 920
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1009


Design Notes

The coding region in the part has been codon optimized for use in E.coli.


Source

Ms2 Bacteriophage

References