Coding

Part:BBa_K3724014

Designed by: Tracey Moyston   Group: iGEM21_Rochester   (2021-10-01)
Revision as of 03:02, 22 October 2021 by Traceymoyston (Talk | contribs) (→‎Usage and Biology)


SO_3468+ribBA+ribE cluster

This gene cluster consists of the genes SO_3468, rib ba and ribe which are genes that encode proteins involved in the riboflavin biosynthesis pathway in Shewanella oneidensis MR-1. This gene cluster makes up a component of the riboflavin synthesis gene cluster (BBa_K3724015).

Usage and Biology

The the riboflavin synthase alpha subunit (SO_3468) which catalyzes the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil from two molecules of 6,7-dimethyl-8-ribityllumazine, the GTP cyclohydrolase-2 (ribA), 3,4-dihydroxy-2-butanone-4-phosphate synthase (ribB) and riboflavin synthase beta subunit (RibE). The latter three proteins catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate , formate and pyrophosphate, catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate and catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate, respectively. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 2373


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