![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3792000
Designed by: Chase Tarillo Group: iGEM21_FSU (2021-09-18)
NodI
Protein possibly involved in transporting chitin across the cell membrane. Expressed with nodC and nodJ by the 2021 FSU team.
To test if the cells were secreting chitin, The FSU 2021 team used the Chitosan Assay Kit (XAN-5126) available from Cell Biolabs. The manual for this kit contains the protocols for converting chitin to chitosan, along with a protocol for detecting chitosan.
Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 150
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Categories
Parameters
None |