Coding

Part:BBa_K3738002:Design

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)
Revision as of 01:41, 18 October 2021 by Emily Hagens (Talk | contribs) (→‎Design Notes)


MS2 Bacteriophage Maturation Protein A with C-Terminal Histidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 836
    Illegal XhoI site found at 919
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 925


Design Notes

This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag has been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.

This part has been codon optimized for use in E.coli.

Source

Source: https://www.uniprot.org/uniprot/P03610

References

Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8