![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4060505:Design
Designed by: Cheng-Ju Lu Group: iGEM21_NYCU-Taipei (2021-10-21)
BphP1 gene
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 760
Illegal PstI site found at 1057 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 760
Illegal PstI site found at 1057 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 416
Illegal XhoI site found at 2166 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 760
Illegal PstI site found at 1057 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 367
Illegal NgoMIV site found at 402
Illegal NgoMIV site found at 1650
Illegal NgoMIV site found at 2152 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1207
Illegal BsaI.rc site found at 2053
Design Notes
The construction of this part is according to igem assembly protocol.
Source
The source of the gene is from Addgene plasmid pKA-207I10.