Composite

Part:BBa_K3763023

Designed by: Guokai Wang   Group: iGEM21_WHU-China   (2021-10-18)
Revision as of 02:24, 22 October 2021 by MAOLIRAN (Talk | contribs)


the expression operator in pET26b+ and CDS of PctA

This part can be induced to express our interested protein Propionicin T1 with native peptide and 6xHis-tag in cytosol,which can be purified using Ni-IMAC.After purification, we can used the extracts to test the inhibition ability of PctA against Propionibacterium acnes.Further more,we designed that the native leader peptide can guide the secretion of PctA in engineered bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 356
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 340


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Categories
Parameters
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